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FLOWMATTERS™ NEWSLETTERS
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Mouse Blood Flow
Transgenic and gene targeting technologies have propelled the development of specific models of cardiovascular pathology in mice. The achievement of successful physiological measurements in mice depends on precise, innovative technology and proven protocols for their use. In a stable, acute murine model, in vivo pressure and high resolution, beat-to-beat blood flow data can be recorded with Transonic micro-flowprobes for calculations of stroke volume, vascular resistance and cardiac pressure/volume relationships.
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Original tracing of ascending aortic blood flow measured by the 1.5PSL Transonic Flowprobe 7 days after implantation in a conscious mouse. Arterial pressure was obtained from a catheter inserted into the abdominal aorta via the femoral artery.
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Flowprobe Suggestions & Application Support
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Vessel
& Body Weight
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Acute
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Chronic
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References
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Aorta abdominal
20-50g
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MA1PRB
1 mm Precision R-Series, back cable exit
60cm cable
CRA10 connector
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MC1.5PSL
1.5 mm Precision S-Series, lateral cable exit
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all |
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Aorta ascending
20-50g
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MA1.5PSL
1.5 mm Precision S-Series, lateral cable exit
60cm cable
CRA10 connector
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MC1.5PSL
1.5 mm Precision S-Series, lateral cable exit
~4cm cable
CA4S connector
surgical protocol
surgical protocol (ppt)
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all
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Aorta descending
20-50g
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MA1PRB
1 mm Precision R-Series, back cable exit
60cm cable
CRA10 connector
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Carotid artery
20-50g
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MA0.5PSB, MA0.7PSB
0.5 / 0.7 mm Precision S-Series, back cable exit
handle
60cm cable
CRA10 connector
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MC0.5PSB, MC0.7PSB
0.5 / 0.7 mm Precision S-Series, back cable exit
~4cm cable
CA4S connector
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Cerebral perfusion
30 g
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Type N (18 gauge)
or
Type M (monofiber)
Laser Doppler Probes
surgical protocol
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Femoral artery
20-50g
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MA0.5PSB
0.5 mm Precision S-Series, back cable exit
handle
60cm cable
CRA10 connector
surgical protocol ppt
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MC0.5PSS
0.5 mm Precision S-Series, side cable exit
~8cm cable
CA4 connector
exercise movie:
8 mg, 15.4 mg
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all
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isolated Heart
20-50g
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ME1PXN, ME2PXN
3/64" / 1/16" ID
Precision XN-Series inline for TS410 module
Langendorff: 2-8 ml/min
working heart: 8-15 ml/min
KREBS or BUFFER
application support
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Hepatic artery
20-50g
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MA0.5PSB
0.5 mm Precision S-Series, back cable exit
handle
60cm cable
CRA10 connector
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all |
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superior Mesenteric artery
20-50g
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MA0.7PSB
0.7 mm Precision S-Series, back cable exit
handle
60cm cable
CRA10 connector
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MC0.7PSB
0.5 / 0.7 mm Precision S-Series, back cable exit
~6cm cable
CA4S connector
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all |
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Portal vein
20-50g
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MA1.5PSL
1.5 mm Precision S-Series, lateral cable exit
60cm cable
CRA10 connector
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all |
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Pulmonary artery
20-50g
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MA1PRB
1 mm Precision R-Series, back cable exit
60cm cable
CRA10 connector
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all |
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Renal artery
20-50g
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MA0.5PSB
0.5 mm Precision S-Series, back cable exit
handle
60cm cable
CRA10 connector
surgical protocol
acute / chronic surgical protocol
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MC0.5PSL
0.5 mm Precision S-Series, lateral cable exit
~6cm cable
CA4S connector
surgical protocol (ppt)
acute / chronic surgical protocol
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all
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Tools & Techniques for Hemodynamic Studies in Mice
This booklet includes pertinent protocols for Pressure Measurements: Mouse arterial catheter construction, Chronic cannulation of the femoral artery, Acute cannulation of the carotid atery; Tissue Perfusion Measurements: Cerebral Perfusion. Use online links for the most current volume flow measurement protocols.Information, tools, techniques for measuring flow in the mouse including:
Pressure
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