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Laser Doppler Support

Protocols, Technical Notes, Videos, and Reprints

 

Support Highlights

Applications

Technique

Tools

Plastic & Reconstructive Surgery

Laser Doppler Tissue Perfusion Studies - Animal Models

Post-op Flap monitoring

Data acquisition

Safety

Sterilizing

Laser Doppler Theory

Reprints

BLF 21 Perfusion-Series Monitors

Meter Electives

Laser Doppler Probes

Accessories (Balancer)

 

Surgical Protocols
dog

mouse

rat

rabbit

sheep

 

Technical Notes

Considerations for Measurement by Laser Doppler Flowmetry

Data Recording from BLF 21 Flowmeters

Implications of the Time Constant Setting on BLF 21 Flowmeters

Selection of Probe Holders for LDF

Laser Doppler Safety

Cleaning & Sterilization of Laser Doppler Probes

Laser Doppler Theory of Operation

Use of TRANSPAC with Pressure (-R) Option

Use of the Monofiber Laser Doppler Probe

 

Video Support (Available Upon Request)

Introduction to Laser Doppler Flowmetry (vi-7)
FASEB'90 Instrumentation Tutorial, Washington, D.C.

  • Introduction to Laser Doppler Flowmetry --A.P. Shepherd
  • Principles and Theory of Measuring Tissue Perfusion by LDF --R.F. Bonner
  • Challenges in Measuring Gastrointestinal Blood Flow by LDF -- J.W. Kiel
  • Studies in the Cutaneous Circulation by LDF --J.M. Johnson
  • Recent and Future Developments in Laser-Doppler Blood Flowmetry --G.Nilsson

Techniques in Laser Doppler Flowmetry (vi-10)
FASEB '92 Instrumentation Tutorial, Anaheim, CA.

  • LDF Measurement of Subpleural Blood Flow in the Dog Lung --T. Hakim
  • Clinical Use of LDF in Cutaneous Circulation and Tissue Transplantation --B. Klitzman
  • Cerebral Blood Flow Measurement in Animals with Laser Doppler --R. Willette
  • LDF as Applied to Myocardial Blood Flow in Dogs --B. Holt

 

Sampling of References

Transonic Systems' DATA BASE of more than 2,000 scientific publications is assessable at SEARCH with hyperlinks to PubMed for immediate perusal of abstracts. The references below represent a brief sampling of publications which cite use of Transonic Laser Doppler flowmeters.

Splanchnic

  • Pig -Tao, W. et al., "Hypertonic Saline/Dextran for Cardiopulmonary Bypass Reduces Gut Tissue Water but Does Not Improve Mucosal Perfusion", J Surg Res; 57: 994. Salzman, A.L. et al., "Endotoxin-Induced Ileal Mucosal Hypermeability in Pigs: Role of Tissue Acidosis," Am J Physiol; 266: G633-G646, 1994.
  • Rat - Yamaguchi, T., "Continuous Monitoring of Gastroduodenal Mucosal Hemodynamics in Rats by Laser-Doppler Flowmetry and Reflectance Spectrophotometry," Gastroenterologia Japonica; 24(6): 619-625, 1989.

Cerebrum

  • Mouse - Curran-Everett, D. et al., "Hypoxia, Hypercapnia, and Hypertension: Their Effects on Pulsatile Cerebral Blood Flow," J App Physiol; 79: 870-878, 1995.

Liver

  • Rat - Kurihara, T. et al., "Relationship between Endothelium and Thrombozane A2 in Rat Liver Microcirculation," Life Sciences; 51: PL 281-285, 1992.

Lung

  • Dog - Hakim, T. et al., "Blood Flow, Volume, and Transit Time in the Pulmonary Microvasculature Using Laser-Doppler," J Appl Physiol; 76(6): p. 2643-2560, 1994.

Pancreas

  • Dog- Kogire, M. et al., "Effects of Intravenous Ethanol on Hepatic and Pancreatic Blood Flow in Dogs," Dig Dis Sci; 33(5): 988.

Kidney

  • Pig - Chiu, A.W. et al., "A Porcine Model for Renal Hemodynamic Study during Laparoscopy," J Surg Res; 60: 61-68, 1994.
    Rat - Guan, Z. et al., "Zaprinast Accelerates Recovery from Established Acute Renal Failure in the Rat," Kid Int; 47, 1569-1575, 1995.

Heart

  • Dog - Hoit, B.D. et al., "Comparative Assessment of Regional Left Atrial Perfusion by Laser Doppler and Radionuclide Microsphere Techniques," Cardiovasc Res; 27(3): 508-514, 1993.
    Pig - Sidi, A., Rush, W., "An Alternative to Radioactive Microspheres for Measuring Regional Myocardial Blood Flow, Part 2: Laser Doppler Perfusion Monitor," J Cardiothor Vasc Anesthes; 10(3): 374-377, 1996.

Cutaneous

  • Rabbit - Smith, T.L., Koman, L.A., "Development of A New Rabbit Ear Model for Longitudinal Study of Digital Pathophysiology," Microsurg; 3: 325-331, 1992.

 

 

 
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