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Effects of Anesthetics on Systemic Hemodynamics in Mice:
Ben J.A. Janssen, Tijl De Celle, Jacques J.M. Debets, Agnieszka E. Brouns, Michael F. Callahan and Thomas L. Smith. American Journal of Physiology Heart and
Circulatory Physiology, 2004 May 20 [Epub ahead of print].
The laboratories of Ben J. A. Janssen, Dept. of Pharmacology & Toxicology, Cardiovascular Research
Institute Maastricht, Universiteit of Maastricht, the Netherlands and Thomas L. Smith, Dept. of Orthopaedic Surgery, Wake Forest University School of Medicine,
Winston-Salem, North Carolina combined efforts and data to examine the cardiovascular effects of four commonly used anesthetic regimens, isoflurane, urethane,
pentobarbital, and ketamine/xylazine in mice. Mice were instrumented with Transonic ascending aorta flowprobes for cardiac output measurements and fully
recovered from surgery. Consicous measurements of cardiac output, stroke volume, heart rate, mean arterial pressure and total peripheral resistance were
made, and compared with measurements made post recovery under general anesthesia. MAP and CO were lowered in all anesthetic regimens.
Isoflurane significantly affected systemic hemodynamics the least. Pentobarbital Na depressed cardiac index (CO normalized to weight) by as much as -40%, and ketamine/xylazine from -37 to -63%. This study provides provocative discussions on the differential hemodynamic effects of anesthetics in the absence of surgery and the dynamic physiologic range of systemic hemodynamics across three strains of conscious mice. The study confirms that isoflurane anesthesia preserves cardiac function better than other anesthetic regimens. The study underscores the importance of choosing an appropriate anesthetic that will not confound study results.
Experimental Biology 2004: Renal Hemodynamics and GFR
The majority of the presentations at the Renal Hemodynamics and GFR Session at the EB conference made
use of Transonic flowmeters to report renal blood flow in a variety of animal models from mice and rats to rabbits and lambs. Scientists from the
some of the most active laboratories studying renal hemodynamics tackled the complex underlying mechanisms of renal blood flow autoregulation, renal vascular
resistance and dilatory responses. In addition to reporting of direct renal blood flow measurements, some groups also utilized Transonic Systems’ laser Doppler
flowmetry to examine superficial renal blood flow or perfusion in the kidney.
Just, A., Olson, J.M., Arendshorst, W.J. (2004) Relative Contribution and Interactions of ET Receptor
Subtypes in the Renal Hemodynamic Response to Endothelin-1. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #504.
Just, A., Arendshorst, W.J. (2004) Pressure-dependent variation of the contribution of myogenic
response and tubuloglomerular feedback to renal blood flow autoregulation. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18
, Abstract #736.
Boffa, J-J., Coffman, T.M., Arendshorst, W.J. Role of Thromboxane A2 in the Regulation os Renal
Hemodynamics in Mice. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #7923.
Hashimoto, S., Hansen, P.B., Huang, Y.G., Briggs, J.P., Schnermann, J.B. The role of adenosine A2a
receptors in the dilatory response to adenosine in mice. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #7047.
Stepp, D.W., Hair, C.D., Pollock, D.M. Increased Reactivity to Angiotensin in the Renal Circulation
of the Obese Zucker Rat. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #1959.
Cupples, W.A., Shi, Y. Nitric Oxide (NO)-Endothelin
(ET) interaction in control of renal blood flow. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #5450.
Zhong, Y., Cupples, W.A., Moore, L., Chon, K. Analysis of renal autoregulatory dynamics using
time-varying transfer and coherence functions. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #3066.
Chappellaz, M.L., Smith, F.G. Dose dependent effects of ANG II on systemic and renal hemodynamics is
conscious lambs. 2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #1129.
Welch, W., Solis, G., Chabrashvili, T., Zhang, Y.J., Xie, H.J., Dennehy, K.J., Aslam, S.J., Marklund,
S.J., Wilcox, C.J. The roles of renal superoxide dismutases (SOD) on the slow pressor effects of angiotensin II.
2004 Experimental Biology meeting abstracts. The FASEB Journal, 18, Abstract #3731.
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