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Anesthetize the mouse with pentobarbital (60 mg/kg IP). To prevent
coagulation, administer heparin (1000 IU/kg) intravenously in the right femoral vein. A cannula is placed in the trachea for ventilation. Make a longitudinal skin and
muscle incision opening the abdomen from the diaphragm to the throat. Cut the diaphragm free from the ribs. Open the thorax following the bone-cartilage border on the left
and right sides parallel to the sternum from the diaphragm cranially to the first rib. Turn the complete anterior thoracic wall upwards over the head to expose the heart.
Remove the pericardium. Separate the ascending aorta from connective tissue and the pulmonary artery using blunt dissection. Preplace a thread around the aorta.
Prepare for insertion of the aortic cannula: Prime the cannula to remove
air bubbles and allow a small stream of perfusate during insertion. Clamp the vena cava above the diaphragm to minimize bleeding. Sprinkle the heart with cold
physiological saline (4º C) so the heart slows down and stops beating. Incise the pulmonary artery to avoid distension of the right ventricle. Incise the aorta as far
cranially as possible and insert the cannula, taking care that the position of the cannula is not too low to impede the aortic valves or the coronary ostia. Tighten the
thread around the end of the cannula. Fully perfuse the heart. Completely isolate and remove the heart for transfer to the Langendorff apparatus.
Transonic Systems' research flowmeters operate inline (flow through)
flowprobes for high resolution volume flow measurements in extracorporeal circuits. Ultrasonic transit time flowprobes can measure blood, buffer solutions and other
non-aerated liquids used in perfused organ studies. A dual channel or dual module flowmeter will operate two 1N flowprobes to simultaneously measure aortic flow and venous
return for cardiac output.
- direct volumetric flow measurement
- high resolution and sensitivity for low flow conditions
- exceptional electrical and zero baseline stability
- measures blood, saline & other non-aerated liquids
References List updated in June 2002
1087A Chen, E.P., Bittner, H.B., Davis, R.D., Van Trigt, P.,
Folz, R.J., "Physiologic Effects of Extracellular Superoxide Dismutase Transgene Overexpression of Myocardial Function after Ischemia and Reperfusion," Journal
of Thoracic and Cardiovascular Surgery, Vol. 115, p. 450-459, 1998. (isolated heart, mouse, 2N probe)
Bittner, H.B., Chen, E.P., Peterseim, D.S., Van Trigt, P.,
"A Work-Performing Heart Preparation for Myocardial Performance Analysis in Murine Hearts," Journal of Surgical Research, Vol 64, p. 57-62, 1996.
Grupp, I.L., Subramaniam, A., Hewett, T.E., Robbins, J.,
Grupp, G., "Comparison of Normal, Hypodynamic and Hyperdynamic Mouse Hearts Using Isolated Work-Performing Heart Preparations," American Journal of Physiology,
Vol 265, p. H1401-1410, 1993.
Lu, W., Grupp, I.L., Harrer, J., Ponniah, S., Grupp, G.,
Duffy, J.J., Doetschman, T., Kranias, E.G., "Targeted Ablation of the Phospholamban Gene Is Associated with Markedly Enhanced Myocardial Contractility and Loss of
-Agoinist Stimulation ," Circulation Research, Vol. 75, p. 401-409, 1994.
Mutchuchamy, M., Grupp, I.L., Grupp, G., O'Toole, B., Kier,
A.B., Boivin, G.P., Neumann, J., Wieczorek, D.F., "Molecular and Physiological Effectts of Overexpressing Striated Muscle -Tropomyosin in the Adult Murine Heart
," The Journal of Biological Chemistry, Vol. 270, No. 51, p. 30593-30603, 1995.Döring, H.J., Dehnert, H., The Isolated Perfused Heart According to Langendorff,
English edition, Biomesstechnick-Verlag, West Germany, 1988, (available from Transonic Systems).
Rev 3/97
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Mouse Myocardial performance
